Main Article Content
Human and animals can accidentally be exposed to heavy metals from the environment such as lead (Pb). Lead may induce oxidative stress. It can increase the production of free radicals, and induces several responses in physiological and biochemical functions of the body. Curcumin, a major component of turmeric, is commonly used as a spice and in traditional medicine. The objective of this research was to evaluate the antioxidant activities of curcumin in mice that were exposed to lead. Research was conducted using twenty five male mice, which were grouped into five treatments: P1 (control), P2 (Pb 75 mg/kg BW), P3 (Pb 150 mg/kg BW), P4 (Pb 75 mg/kg BW + curcumin 20 ppm), P5 (Pb 150 mg/kg BW + curcumin 20 ppm). The results showed that antioxidant activities of curcumin was very strong with an IC-50 was 9.0 ppm. Pb exposure increased MDA level (17.143â€“17.891 ÂµM) and Pb level in the liver (0.070â€“0.071 mg/kg BW). Administration of curcumin 20 ppm have the potential to reduced MDA level (14.592â€“15.714 ÂµM) and reduce Pb levels (0.035â€“0.038 mg/kg BW).
Transfer of Copyrights
- In the event of publication of the manuscript entitled [INSERT MANUSCRIPT TITLE AND REF NO.] in the Malaysian Journal of Science, I hereby transfer copyrights of the manuscript title, abstract and contents to the Malaysian Journal of Science and the Faculty of Science, University of Malaya (as the publisher) for the full legal term of copyright and any renewals thereof throughout the world in any format, and any media for communication.
Conditions of Publication
- I hereby state that this manuscript to be published is an original work, unpublished in any form prior and I have obtained the necessary permission for the reproduction (or am the owner) of any images, illustrations, tables, charts, figures, maps, photographs and other visual materials of whom the copyrights is owned by a third party.
- This manuscript contains no statements that are contradictory to the relevant local and international laws or that infringes on the rights of others.
- I agree to indemnify the Malaysian Journal of Science and the Faculty of Science, University of Malaya (as the publisher) in the event of any claims that arise in regards to the above conditions and assume full liability on the published manuscript.
- Reviewers must treat the manuscripts received for reviewing process as confidential. It must not be shown or discussed with others without the authorization from the editor of MJS.
- Reviewers assigned must not have conflicts of interest with respect to the original work, the authors of the article or the research funding.
- Reviewers should judge or evaluate the manuscripts objective as possible. The feedback from the reviewers should be express clearly with supporting arguments.
- If the assigned reviewer considers themselves not able to complete the review of the manuscript, they must communicate with the editor, so that the manuscript could be sent to another suitable reviewer.
Copyright: Rights of the Author(s)
- Effective 2007, it will become the policy of the Malaysian Journal of Science (published by the Faculty of Science, University of Malaya) to obtain copyrights of all manuscripts published. This is to facilitate:
(a) Protection against copyright infringement of the manuscript through copyright breaches or piracy.
(b) Timely handling of reproduction requests from authorized third parties that are addressed directly to the Faculty of Science, University of Malaya.
- As the author, you may publish the fore-mentioned manuscript, whole or any part thereof, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
You may produce copies of your manuscript, whole or any part thereof, for teaching purposes or to be provided, on individual basis, to fellow researchers.
- You may include the fore-mentioned manuscript, whole or any part thereof, electronically on a secure network at your affiliated institution, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
- You may include the fore-mentioned manuscript, whole or any part thereof, on the World Wide Web, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
- In the event that your manuscript, whole or any part thereof, has been requested to be reproduced, for any purpose or in any form approved by the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers), you will be informed. It is requested that any changes to your contact details (especially e-mail addresses) are made known.
Copyright: Role and responsibility of the Author(s)
- In the event of the manuscript to be published in the Malaysian Journal of Science contains materials copyrighted to others prior, it is the responsibility of current author(s) to obtain written permission from the copyright owner or owners.
- This written permission should be submitted with the proof-copy of the manuscript to be published in the Malaysian Journal of Science
Amriani, Hendrarto B., and Hadiyarto A., (2011). Bioakumulasi Logam Berat Timbal (Pb) Dan Seng (Zn) Pada Kerang Darah (Anadara granosa L.) Dan Kerang Bakau (Polymesoda bengalensis L.) Di Perairan Teluk Kendari, Jurnal Ilmu Lingkungan 9(2): 45-50.
Faix S., Z. Faixova Z., Boldizarova K., and Javorsky P., (2005). The Effect of Long-Term High Heavy Metal Intake on Lipid Peroxidation of Gastrointestinal Tissue in Sheep, VeterinÃ¡rni Medicina â€“ Czech 50(9): 401â€“405.
Fauziah S.H., NurHajar W.S., and Agamuthu P., (2011). Heavy Metal Accumulation in Ipomoea reptans and Helianthus annuus, Malaysian Journal of Science 30(1): 36-44.
Flora G., Gupta D., and Tiwari A., (2012). Toxicity of Lead: A Review With Recent Updates, Interdisciplinary Toxicology 5(2): 47-58.
Fuente H.D., Perez D.P., Baranda L., Barriga F.D., Alanis V.S, Layseca E., and Amaro R.G., (2002). Effect of Arsenic, Cadmium, and Lead on The Induction Apoptosis of Normal Human Mononuclear Cells, Clinical & Experimental Immunology 129: 69-77.
Hayati A., Sugiharto, Abdizen M., Solikha B.M., Maulidyah N., Tiantono N., Seta A.R., Widyana H., Restinastiti I., Ziky D., and Winarni D., (2017). Bioaccumulation of Heavy Metals in Fish (Barbodes sp.) Tissues in the Brantas River, Indonesia, Journal of Applied Environmental and Biological Sciences 7(3): 139-143.
Khan H., Ullah H., and Nabavi S.M., (2019). Mechanistic Insights of Hepatoprotective Effects of Curcumin: Therapeutic Updates and Future Prospects, Food and Chemical Toxicology 124: 182â€“191.
Lee K., Aziz F.H., Syahida A., Abas F., Shaari K., Israf D.A., and Lajis N.H., (2009). Synthesis and Biological Evaluation of Curcumin-Like Diarylpentanoid Analogues for Anti-Inflammatory, Antioxidant and Anti-Tyrosinase Activities, European Journal of Medicinal Chemistry 44(8): 3195-3200.
Liu Z., He C., Chen M., Yang S., Li J., Lin Y., Deng Y., Li N., Guo Y., Yu P., and Li X., (2018). The Effects of Lead and Aluminum Exposure on Congenital Heart Disease and The Mechanism of Oxidative Stress, Reproductive Toxicology 81: 93â€“98.
Molyneux P., (2004). The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity, Songklanakarin Journal of Sciences and Technology 26(2): 211-219.
Nimse S.B., and Pal D., (2015). Free Radicals, Natural Antioxidants, and Their Reaction Mechanisms, RSC Advances 5: 27986-28006.
Priyadarsini K. I., Maity D. K., Naik G. H., Kumar M. S., Unnikrishnan M. K., Satav J. G., and Mohan H. (2003). Role of Phenolic O-H and Methylene Hydrogen on the Free Radical Reactions and Antioxidant Activity of Curcumin, Free Radical Biology and Medicine, 35(5): 475-484.
Raj S., and Shankaran D.R., (2016). Curcumin Based Biocompatible Nano-fibers For Lead Ion Detection, Sensors and Actuators B 226: 318â€“325.
Shah F.C., and Jain N.K., (2016). Ameliorative Action of Synthetic and Herbal Antioxidants on Lead Induced Hepatotoxicity: An In Vitro Study, Asian Journal of Pharmaceutical and Clinical Research 9(2) : 364â€“370.
Sharma S., and Singh B., (2014). Effects of Acute and Chronic Lead Exposure on Kidney Lipid Peroxidation and Antioxidant Enzyme Activities in BALB-C Mice (Mus musculus), International Journal of Science and Research 3(9): 1564-1566.
Sugiharto, Arbakariya A., Syahida A., and Muhajir H., (2013). Properties of Curcumin: Assay of Tyrosinase Activities, Proceeding of 4th ICOWOBASâ€“RAFSS, UTMâ€“Malaysia, 80â€“82.
Sugiharto, Arbakariya A., Syahida A., and Muhajir H., (2016). Properties of Kojic Acid and Curcumin: Assay on Cell B16-F1, 5th ICOWOBAS 2015, AIP Conference Proceedings 1718, 060006; https://doi.org/10.1063/1.4943328
Sugiharto, Win Darmanto, and Sri Puji A.W., (2018). Antioxidant Activity of Curcumin on Blood Serum Assay and Liver Cells of Mice, Research Report, Faculty of Sciences and Technology, Airlangga University.
Ugya A.Y., and Imam T.S., (2017). Temporal Heavy Metal Variation in Vegetables Sampled at Kasuwan Mata, Kaduna Metropolis, Nigeria, Malaysian Journal of Science 36(2): 63-74.
Xu J., Lian L.J., Wu C., Wang X.F., Fu W.Y., and Xu L. H., (2008). Lead Induces Oxidative Stress, DNA Damage and Alteration of p53, Bax and Bcl-2 Expressions in Mice, Food and Chemical Toxicology 46(5): 1488-1494.