Technical Improvement in DNA Preparation for Pulsed—Field Gel Electrophoresis

Authors

  • Thong Kwai Lin
  • Lai Kin Seng
  • Goh Yee Ling

Abstract

We report a rapid pulsed-field electrophoresis (PFGE) protocol for subtyping $Salmonella$ isolates. Modifications were made to reduce the number of steps and duration of proteolysis in our original protocol for the preparation of intact, genomic DNA for PFGE analysis. Comparison of PFGE patterns by independent researchers showed that subtyping results obtained using this modified, rapid protocol were highly reproducible.

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Published

28-08-2003

How to Cite

Kwai Lin, T., Kin Seng, L., & Yee Ling, G. (2003). Technical Improvement in DNA Preparation for Pulsed—Field Gel Electrophoresis. Malaysian Journal of Science (MJS), 22(2), 55–59. Retrieved from https://mjs.um.edu.my/index.php/MJS/article/view/8516

Issue

Vol. 22 No. 2 (2003)

Section

Original Articles

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