ANTIOXIDANT ACTIVITIES OF CURCUMIN TO MDA BLOOD SERUM CONCENTRATION AND LEAD LEVELS IN LIVER OF MICE
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Published:
Nov 30, 2019
Keywords:
Antioxidant Curcumin Lead Liver MDA
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Abstract
Human and animals can accidentally be exposed to heavy metals from the environment such as lead (Pb). Lead may induce oxidative stress. It can increase the production of free radicals, and induces several responses in physiological and biochemical functions of the body. Curcumin, a major component of turmeric, is commonly used as a spice and in traditional medicine. The objective of this research was to evaluate the antioxidant activities of curcumin in mice that were exposed to lead. Research was conducted using twenty five male mice, which were grouped into five treatments: P1 (control), P2 (Pb 75 mg/kg BW), P3 (Pb 150 mg/kg BW), P4 (Pb 75 mg/kg BW + curcumin 20 ppm), P5 (Pb 150 mg/kg BW + curcumin 20 ppm). The results showed that antioxidant activities of curcumin was very strong with an IC-50 was 9.0 ppm. Pb exposure increased MDA level (17.143–17.891 µM) and Pb level in the liver (0.070–0.071 mg/kg BW). Administration of curcumin 20 ppm have the potential to reduced MDA level (14.592–15.714 µM) and reduce Pb levels (0.035–0.038 mg/kg BW).
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S., S., Darmanto, W., Wahyuningsih, S. P. A., Hayati, A., Martha, Y., Olivia, P., & Putri, K. (2019). ANTIOXIDANT ACTIVITIES OF CURCUMIN TO MDA BLOOD SERUM CONCENTRATION AND LEAD LEVELS IN LIVER OF MICE. Malaysian Journal of Science, 38(Sp3), 21–29. https://doi.org/10.22452/mjs.sp2019no3.3
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UMInd2018 (Published)
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Raj S., and Shankaran D.R., (2016). Curcumin Based Biocompatible Nano-fibers For Lead Ion Detection, Sensors and Actuators B 226: 318–325.
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Xu J., Lian L.J., Wu C., Wang X.F., Fu W.Y., and Xu L. H., (2008). Lead Induces Oxidative Stress, DNA Damage and Alteration of p53, Bax and Bcl-2 Expressions in Mice, Food and Chemical Toxicology 46(5): 1488-1494.
Amriani, Hendrarto B., and Hadiyarto A., (2011). Bioakumulasi Logam Berat Timbal (Pb) Dan Seng (Zn) Pada Kerang Darah (Anadara granosa L.) Dan Kerang Bakau (Polymesoda bengalensis L.) Di Perairan Teluk Kendari, Jurnal Ilmu Lingkungan 9(2): 45-50.
Faix S., Z. Faixova Z., Boldizarova K., and Javorsky P., (2005). The Effect of Long-Term High Heavy Metal Intake on Lipid Peroxidation of Gastrointestinal Tissue in Sheep, Veterinárni Medicina – Czech 50(9): 401–405.
Fauziah S.H., NurHajar W.S., and Agamuthu P., (2011). Heavy Metal Accumulation in Ipomoea reptans and Helianthus annuus, Malaysian Journal of Science 30(1): 36-44.
Flora G., Gupta D., and Tiwari A., (2012). Toxicity of Lead: A Review With Recent Updates, Interdisciplinary Toxicology 5(2): 47-58.
Fuente H.D., Perez D.P., Baranda L., Barriga F.D., Alanis V.S, Layseca E., and Amaro R.G., (2002). Effect of Arsenic, Cadmium, and Lead on The Induction Apoptosis of Normal Human Mononuclear Cells, Clinical & Experimental Immunology 129: 69-77.
Hayati A., Sugiharto, Abdizen M., Solikha B.M., Maulidyah N., Tiantono N., Seta A.R., Widyana H., Restinastiti I., Ziky D., and Winarni D., (2017). Bioaccumulation of Heavy Metals in Fish (Barbodes sp.) Tissues in the Brantas River, Indonesia, Journal of Applied Environmental and Biological Sciences 7(3): 139-143.
Khan H., Ullah H., and Nabavi S.M., (2019). Mechanistic Insights of Hepatoprotective Effects of Curcumin: Therapeutic Updates and Future Prospects, Food and Chemical Toxicology 124: 182–191.
Lee K., Aziz F.H., Syahida A., Abas F., Shaari K., Israf D.A., and Lajis N.H., (2009). Synthesis and Biological Evaluation of Curcumin-Like Diarylpentanoid Analogues for Anti-Inflammatory, Antioxidant and Anti-Tyrosinase Activities, European Journal of Medicinal Chemistry 44(8): 3195-3200.
Liu Z., He C., Chen M., Yang S., Li J., Lin Y., Deng Y., Li N., Guo Y., Yu P., and Li X., (2018). The Effects of Lead and Aluminum Exposure on Congenital Heart Disease and The Mechanism of Oxidative Stress, Reproductive Toxicology 81: 93–98.
Molyneux P., (2004). The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity, Songklanakarin Journal of Sciences and Technology 26(2): 211-219.
Nimse S.B., and Pal D., (2015). Free Radicals, Natural Antioxidants, and Their Reaction Mechanisms, RSC Advances 5: 27986-28006.
Priyadarsini K. I., Maity D. K., Naik G. H., Kumar M. S., Unnikrishnan M. K., Satav J. G., and Mohan H. (2003). Role of Phenolic O-H and Methylene Hydrogen on the Free Radical Reactions and Antioxidant Activity of Curcumin, Free Radical Biology and Medicine, 35(5): 475-484.
Raj S., and Shankaran D.R., (2016). Curcumin Based Biocompatible Nano-fibers For Lead Ion Detection, Sensors and Actuators B 226: 318–325.
Shah F.C., and Jain N.K., (2016). Ameliorative Action of Synthetic and Herbal Antioxidants on Lead Induced Hepatotoxicity: An In Vitro Study, Asian Journal of Pharmaceutical and Clinical Research 9(2) : 364–370.
Sharma S., and Singh B., (2014). Effects of Acute and Chronic Lead Exposure on Kidney Lipid Peroxidation and Antioxidant Enzyme Activities in BALB-C Mice (Mus musculus), International Journal of Science and Research 3(9): 1564-1566.
Sugiharto, Arbakariya A., Syahida A., and Muhajir H., (2013). Properties of Curcumin: Assay of Tyrosinase Activities, Proceeding of 4th ICOWOBAS–RAFSS, UTM–Malaysia, 80–82.
Sugiharto, Arbakariya A., Syahida A., and Muhajir H., (2016). Properties of Kojic Acid and Curcumin: Assay on Cell B16-F1, 5th ICOWOBAS 2015, AIP Conference Proceedings 1718, 060006; https://doi.org/10.1063/1.4943328
Sugiharto, Win Darmanto, and Sri Puji A.W., (2018). Antioxidant Activity of Curcumin on Blood Serum Assay and Liver Cells of Mice, Research Report, Faculty of Sciences and Technology, Airlangga University.
Ugya A.Y., and Imam T.S., (2017). Temporal Heavy Metal Variation in Vegetables Sampled at Kasuwan Mata, Kaduna Metropolis, Nigeria, Malaysian Journal of Science 36(2): 63-74.
Xu J., Lian L.J., Wu C., Wang X.F., Fu W.Y., and Xu L. H., (2008). Lead Induces Oxidative Stress, DNA Damage and Alteration of p53, Bax and Bcl-2 Expressions in Mice, Food and Chemical Toxicology 46(5): 1488-1494.