STERILIZABLE MINIATURE BIOREACTOR PLATFORM FOR ANAEROBIC FERMENTATION PROCESS
Article Sidebar
Published:
Feb 28, 2021
Keywords:
Miniature bioreactor anaerobic fermentation scale down miniaturization S.cerevisiae
Main Article Content
Abstract
This paper presents the establishment of a miniature bioreactor platform for anaerobic microbial fermentation processes. It is made from a universal glass bottle and has a working volume of 16 mL. Reactor features included mixing via magnetic stirrer, temperature control via electrical heater and cells optical density (OD) sensing. All sensors and actuators integrated into the reactor were operated using LabVIEWTM (National Instrument, TX, US). The top lid of the bottle was modified to include a 3mm poly(methylmethacrylate) (PMMA) polymer layer where it was machined to provide spaces for fluidic ports and integration of sensors. Each reactor components were sterilized prior to conducting fermentation experiments. Main body of the reactor was made of glass and it was sterilized using the standard heat sterilization method (121oC for 15 minutes) where else other components were sterilized by exposure to UV light for 2 hours. A series of anaerobic fermentation experiments were conducted in batch mode using S.cerevisiae to evaluate the workability of the system. Fermentation experiments were conducted using inoculum concentration of 2 g∙L-1 and starting glucose concentration between 10 g∙L-1 and 20 g∙L-1. In every experiment, mixing was set to operate at 400 rpm and temperature was adjusted to 30 ± 2oC. Experiments were carried out until stationary phase was attained. Under these conditions, the best fermentation profile was obtained with glucose concentration of 20 /L where cell specific growth rate was found to be about 0.28 h-1. Bench marking step was also performed where results attained in a miniature bioreactor platform were comparable with the one attained using a 50 mL flask.
Downloads
Download data is not yet available.
Article Details
How to Cite
Arriafdi, Z., Ramachendrin, A., & Zainal Alam, M. N. H. (2021). STERILIZABLE MINIATURE BIOREACTOR PLATFORM FOR ANAEROBIC FERMENTATION PROCESS. Malaysian Journal of Science, 40(1), 22–33. https://doi.org/10.22452/https://doi.org/10.22452/mjs.vol40no1.2
Section
Original Articles
Transfer of Copyrights
- In the event of publication of the manuscript entitled [INSERT MANUSCRIPT TITLE AND REF NO.] in the Malaysian Journal of Science, I hereby transfer copyrights of the manuscript title, abstract and contents to the Malaysian Journal of Science and the Faculty of Science, University of Malaya (as the publisher) for the full legal term of copyright and any renewals thereof throughout the world in any format, and any media for communication.
Conditions of Publication
- I hereby state that this manuscript to be published is an original work, unpublished in any form prior and I have obtained the necessary permission for the reproduction (or am the owner) of any images, illustrations, tables, charts, figures, maps, photographs and other visual materials of whom the copyrights is owned by a third party.
- This manuscript contains no statements that are contradictory to the relevant local and international laws or that infringes on the rights of others.
- I agree to indemnify the Malaysian Journal of Science and the Faculty of Science, University of Malaya (as the publisher) in the event of any claims that arise in regards to the above conditions and assume full liability on the published manuscript.
Reviewer’s Responsibilities
- Reviewers must treat the manuscripts received for reviewing process as confidential. It must not be shown or discussed with others without the authorization from the editor of MJS.
- Reviewers assigned must not have conflicts of interest with respect to the original work, the authors of the article or the research funding.
- Reviewers should judge or evaluate the manuscripts objective as possible. The feedback from the reviewers should be express clearly with supporting arguments.
- If the assigned reviewer considers themselves not able to complete the review of the manuscript, they must communicate with the editor, so that the manuscript could be sent to another suitable reviewer.
Copyright: Rights of the Author(s)
- Effective 2007, it will become the policy of the Malaysian Journal of Science (published by the Faculty of Science, University of Malaya) to obtain copyrights of all manuscripts published. This is to facilitate:
- Protection against copyright infringement of the manuscript through copyright breaches or piracy.
- Timely handling of reproduction requests from authorized third parties that are addressed directly to the Faculty of Science, University of Malaya.
- As the author, you may publish the fore-mentioned manuscript, whole or any part thereof, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given. You may produce copies of your manuscript, whole or any part thereof, for teaching purposes or to be provided, on individual basis, to fellow researchers.
- You may include the fore-mentioned manuscript, whole or any part thereof, electronically on a secure network at your affiliated institution, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
- You may include the fore-mentioned manuscript, whole or any part thereof, on the World Wide Web, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
- In the event that your manuscript, whole or any part thereof, has been requested to be reproduced, for any purpose or in any form approved by the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers), you will be informed. It is requested that any changes to your contact details (especially e-mail addresses) are made known.
Copyright: Role and responsibility of the Author(s)
- In the event of the manuscript to be published in the Malaysian Journal of Science contains materials copyrighted to others prior, it is the responsibility of current author(s) to obtain written permission from the copyright owner or owners.
- This written permission should be submitted with the proof-copy of the manuscript to be published in the Malaysian Journal of Science
References
Blose, K. J., Krawiec, J. T., Weinbaum, J. S., and Vorp, D. A. (2014). Bioreactors for tissue engineering purposes A2—Orlando: pp177–185.
Bolic, A. (2018). Development and application of a milliliter-scale bioreactor for continuous microbial
cultivations. Thesis of Doctoral Degree, Denmark Technical University.
Bolic, A., Larsson, H., Hugelier, S., Lantz, A.E., Krühne, U., and Gernaey, K.V. (2016). A flexible well-mixed milliliter-scale reactor with high oxygen transfer rate for microbial cultivations. Chemical Engineering Journal. 303, 655–666.
Halimoon, H., Hussain, A., Kouzani, A., & Alam, M. (2016). Aerobic fermentation of Saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS) polymers. Sains Malaysiana, 45(6), 969-976.
Kheradmandnia, S., Hashemi-Najafabadi, S., Shojaosadati, S., Mousavi, S., and Malek Khosravi, K. (2014). Development of parallel miniature bubble column bioreactors for fermentation process. Journal of Chemical Technology & Biotechnology, 90(6), 1051-1061.
Kirk, T. and Szita, N. (2013). Oxygen transfer characteristics of miniaturized bioreactor systems. Biotechnology and Bioengineering, 110(4), 1005-1019.
Krakau, K. (2016). Standard Operating Procedures for a Single-Use Fermenter. Thesis of Bachelor Degree, Technical University of Denmark.
Najafpour, G. (2007). Bioreactor Design. Biochemical Engineering and Biotechnology, pp.142-169.
Panatto, D., Amicizia, D., Bragazzi, N., Rizzitelli, E., Tramalloni, D., Valle, I. and Gasparini,R. (2015) ‘Human Papillomavirus Vaccine. Advances in
Protein Chemistry and Structural Biology’, 231-322.
Schäpper, D., Zainal Alam, M.N.H., Szita, N., Lantz, A.E. & Gernaey, K.V. 2009. Application of microbioreactors in fermentation process development: A review. Analytical and Bioanalytical Chemistry 395(3): 679-695.
Schmideder, A., Severin, T., Cremer, J. and Weuster-Botz, D. (2015). A novel milliliter-scale chemostat system for parallel cultivation of microorganisms in stirred-tank bioreactors. Journal of Biotechnology, 210, .19-24.
Zainal Alam, M., Jaya Kumar, J., John Whyte, D., Doeven, E., & Kouzani, A. (2018). A portable sensor for cell optical density measurement in microfluidic chips. Measurement and Control, 51(7-8), 213-222.
Zainal Alam, M., Schäpper, D. and Gernaey, K. (2010). Embedded resistance wire as a heating element for temperature control in microbioreactors. Journal of Micromechanics and Microengineering, 20(5): 055014.
Zainal Alam, M. N. H., Schäpper, D., and Gernaey, K. V. (2012). ‘Establishment of a Gaseous pH concept in microbioreactors’. International Conference on Food Engineering & Biotechnology (3rd ICFEB), Sepang, Malaysia. ISSN: 2010-4618. Vol3: pg 228-234.
Zelenev, V.V., van Bruggen, A.H.C., and Semenov, A.M. (2005). Modeling wave-like dynamics of oligotrophic and copiotrophic bacteria along wheat roots in response to nutrient input from a growing root tip. Ecological Modelling. 188, 404 – 417.
Bolic, A. (2018). Development and application of a milliliter-scale bioreactor for continuous microbial
cultivations. Thesis of Doctoral Degree, Denmark Technical University.
Bolic, A., Larsson, H., Hugelier, S., Lantz, A.E., Krühne, U., and Gernaey, K.V. (2016). A flexible well-mixed milliliter-scale reactor with high oxygen transfer rate for microbial cultivations. Chemical Engineering Journal. 303, 655–666.
Halimoon, H., Hussain, A., Kouzani, A., & Alam, M. (2016). Aerobic fermentation of Saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS) polymers. Sains Malaysiana, 45(6), 969-976.
Kheradmandnia, S., Hashemi-Najafabadi, S., Shojaosadati, S., Mousavi, S., and Malek Khosravi, K. (2014). Development of parallel miniature bubble column bioreactors for fermentation process. Journal of Chemical Technology & Biotechnology, 90(6), 1051-1061.
Kirk, T. and Szita, N. (2013). Oxygen transfer characteristics of miniaturized bioreactor systems. Biotechnology and Bioengineering, 110(4), 1005-1019.
Krakau, K. (2016). Standard Operating Procedures for a Single-Use Fermenter. Thesis of Bachelor Degree, Technical University of Denmark.
Najafpour, G. (2007). Bioreactor Design. Biochemical Engineering and Biotechnology, pp.142-169.
Panatto, D., Amicizia, D., Bragazzi, N., Rizzitelli, E., Tramalloni, D., Valle, I. and Gasparini,R. (2015) ‘Human Papillomavirus Vaccine. Advances in
Protein Chemistry and Structural Biology’, 231-322.
Schäpper, D., Zainal Alam, M.N.H., Szita, N., Lantz, A.E. & Gernaey, K.V. 2009. Application of microbioreactors in fermentation process development: A review. Analytical and Bioanalytical Chemistry 395(3): 679-695.
Schmideder, A., Severin, T., Cremer, J. and Weuster-Botz, D. (2015). A novel milliliter-scale chemostat system for parallel cultivation of microorganisms in stirred-tank bioreactors. Journal of Biotechnology, 210, .19-24.
Zainal Alam, M., Jaya Kumar, J., John Whyte, D., Doeven, E., & Kouzani, A. (2018). A portable sensor for cell optical density measurement in microfluidic chips. Measurement and Control, 51(7-8), 213-222.
Zainal Alam, M., Schäpper, D. and Gernaey, K. (2010). Embedded resistance wire as a heating element for temperature control in microbioreactors. Journal of Micromechanics and Microengineering, 20(5): 055014.
Zainal Alam, M. N. H., Schäpper, D., and Gernaey, K. V. (2012). ‘Establishment of a Gaseous pH concept in microbioreactors’. International Conference on Food Engineering & Biotechnology (3rd ICFEB), Sepang, Malaysia. ISSN: 2010-4618. Vol3: pg 228-234.
Zelenev, V.V., van Bruggen, A.H.C., and Semenov, A.M. (2005). Modeling wave-like dynamics of oligotrophic and copiotrophic bacteria along wheat roots in response to nutrient input from a growing root tip. Ecological Modelling. 188, 404 – 417.