TISSUE SPECIFIC EXPRESSION OF CHALCONE SYNTHASE (CHS) TRANSCRIPTS AND OVEREXPRESSION OF BrCHS IN CELL SUSPENSION CULTURES OF BOESENBERGIA ROTUNDA.

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Nurnadiah binti Roslan Teh Ser Huy Wong Sher Ming Norzulaani Khalid Zulqarnain Mohamed

Abstract

Abstract


Fingerroot (Boesenbergia rotunda) is a monocot plant from Zingiberaceae family, and it has been used as food, spices and herbal remedy for over 2000 years. In some Asian countries, it is commonly consumed as a traditional meal or used as a spice ingredient and traditional medicine. Its ethnomedicinal properties derived from rhizome extracts have broadly drawn attention to further explore its medicinal and health values. Recently, the compounds extracted from its rhizome have been studied, and the findings showed that it posed anti-cancer, anti-microbial, anti-viral and anti-inflammatory properties. The bioactive compounds from rhizome extracts are plant secondary metabolites synthesized from the flavonoid biosynthesis pathway by a number of enzymes. In most plants, chalcone synthase (CHS) is one of the key enzymes that initiate the flavonoid biosynthesis pathway. In this study, at least four to five copies of BrCHS transcripts were successfully detected and predominantly expressed in a tissue-specific manner (leaves, flowers, rhizomes and cell suspension cultures). Later, BrCHS2 transcript isolated from the rhizomes was chosen to be introduced into B. rotunda cell suspension culture to observe the regulation of transgene expression in the plant pathways.

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How to Cite
ROSLAN, Nurnadiah binti et al. TISSUE SPECIFIC EXPRESSION OF CHALCONE SYNTHASE (CHS) TRANSCRIPTS AND OVEREXPRESSION OF BrCHS IN CELL SUSPENSION CULTURES OF BOESENBERGIA ROTUNDA.. Malaysian Journal of Science, [S.l.], v. 39, n. 2, p. 92-110, june 2020. ISSN 2600-8688. Available at: <https://mjs.um.edu.my/article/view/17799>. Date accessed: 12 aug. 2020. doi: https://doi.org/10.22452/mjs.vol39no2.6.
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