ISOLATION, CHARACTERIZATION AND ANTIBACTERIAL SREENING OF ANTIBIOTICS PRODUCED FROM STREPTOMYCES ISOLATED FROM DUMPSITE SOILS IN ILORIN, NORTH CENTRAL NIGERIA

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Risikat Nike Ahmed
Mercy Oluwaseyi Bamigboye
Kamoldeen Abiodun Ajijolakewu
Sheriffdeen Olakunle Idris
Nimat Toyosi Ajide Bamigboye

Abstract

Abstract


Antibiotics are one of the most exploited metabolites produced by soil actinomycetes. This study isolated fifteen actinomycetes (A1 – A15) from dumpsite soils within Ilorin metropolis & screened them for antibacterial activity. Isolates were identified with morphology & biochemical characteristics.Their  activity against clinical bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae & Staphylococcus aureus) was determined. Their bioactive metabolites after fermentation was assayed for  by agar well diffusion & compared with reference antibiotics. Molecular analysis of two most active isolates was done. The most active metabolite was subjected to GCMS. All isolates were identified as Streptomyces, 7 isolates exhibited activity against 3 test bacteria with highest (10.5mm±0.35) by Streptomyces griseoplanus while S. aureus demonstrated total resistance to all isolates. Secondary activity revealed 7 of the metabolites as effective against the bacteria  with highest activity (28.5±1.04mm) by S. sparsogenes showing similar activity with Cefuroxime (28.01±0.01mm). The two most active isolates  were Streptomyces griseoplanus strain NR725RL-ISP 5009 & Streptomyces sparsogenes strain NBRC 1308616S & RNA nucleotide similarity revealed close phylogenic relationship. GC-MS revealed10 compounds in S. sparsogenes strain NBRC 1308616S metabolite. Streptomyces from dumpsite soils within  Ilorin produced bioactive compounds against  clinical test bacteria and thus could be  potential sources of antibiotics.


 

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